primary normal huc Search Results


primary normal huc  (ScienCell)
90
ScienCell primary normal huc
(A) Schematic representation of experimental conditions including non-irradiated, uniformly-irradiated and shielding protocols where 50% of the slide was exposed and 50% shielded with MCP alloy. Cells were fixed and processed for 53BP1-immunofluorescence, one hour following irradiation. Micrographs <t>of</t> <t>urothelial</t> cancer T24 cells labelled with anti-53BP1 (green) to label nuclear DNA double stand break foci and DAPI to counterstain nuclei are shown for each experimental condition. (B) Summary graph of mean foci/cell in T24 cells (N=3), measured from confocal z-stacks in Volocity software. There were significantly more foci in shielded cells 0.5mm from the shield than in non-irradiated controls, indicative of a bystander effect (p=0.019). (C) Summary data for HT1376 cancer cells (N=3) where there was no difference in the mean foci/cell between non-irradiated controls and shielded cells. (D) Summary data for normal urothelial <t>HUC</t> (N=3). Mean foci/cell was significantly greater in shielded cells in the 0-5mm (p=0.005) and 5-10mm (p=0.04) analysis regions from the shield. * denotes p<0.05, ** denotes p<0.01
Primary Normal Huc, supplied by ScienCell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A) Schematic representation of experimental conditions including non-irradiated, uniformly-irradiated and shielding protocols where 50% of the slide was exposed and 50% shielded with MCP alloy. Cells were fixed and processed for 53BP1-immunofluorescence, one hour following irradiation. Micrographs of urothelial cancer T24 cells labelled with anti-53BP1 (green) to label nuclear DNA double stand break foci and DAPI to counterstain nuclei are shown for each experimental condition. (B) Summary graph of mean foci/cell in T24 cells (N=3), measured from confocal z-stacks in Volocity software. There were significantly more foci in shielded cells 0.5mm from the shield than in non-irradiated controls, indicative of a bystander effect (p=0.019). (C) Summary data for HT1376 cancer cells (N=3) where there was no difference in the mean foci/cell between non-irradiated controls and shielded cells. (D) Summary data for normal urothelial HUC (N=3). Mean foci/cell was significantly greater in shielded cells in the 0-5mm (p=0.005) and 5-10mm (p=0.04) analysis regions from the shield. * denotes p<0.05, ** denotes p<0.01

Journal: Oncotarget

Article Title: Dual effects of radiation bystander signaling in urothelial cancer: purinergic-activation of apoptosis attenuates survival of urothelial cancer and normal urothelial cells

doi: 10.18632/oncotarget.21995

Figure Lengend Snippet: (A) Schematic representation of experimental conditions including non-irradiated, uniformly-irradiated and shielding protocols where 50% of the slide was exposed and 50% shielded with MCP alloy. Cells were fixed and processed for 53BP1-immunofluorescence, one hour following irradiation. Micrographs of urothelial cancer T24 cells labelled with anti-53BP1 (green) to label nuclear DNA double stand break foci and DAPI to counterstain nuclei are shown for each experimental condition. (B) Summary graph of mean foci/cell in T24 cells (N=3), measured from confocal z-stacks in Volocity software. There were significantly more foci in shielded cells 0.5mm from the shield than in non-irradiated controls, indicative of a bystander effect (p=0.019). (C) Summary data for HT1376 cancer cells (N=3) where there was no difference in the mean foci/cell between non-irradiated controls and shielded cells. (D) Summary data for normal urothelial HUC (N=3). Mean foci/cell was significantly greater in shielded cells in the 0-5mm (p=0.005) and 5-10mm (p=0.04) analysis regions from the shield. * denotes p<0.05, ** denotes p<0.01

Article Snippet: Urothelial carcinoma cells T24 (McCoy’s 5A) [ ], HT1376 MEM) [ ], immortalised normal urothelial cells SV-HUC (F12K) [ ], were purchased from ATCC; primary normal HUC (Urothelial Cell Medium) were obtained from ScienCell Research Laboratories.

Techniques: Irradiation, Immunofluorescence, Software

Summary schematic diagram of the radiation-bystander effect that is common to urothelial cancer (T24) cells and normal urothelium (SV-HUC). Directly irradiated cells signal to bystander cells activating pro-apoptotic signaling mechanisms and causing DNA double stand break damage resulting in decreased cell survival. The bystander effect studied here was mediated by ATP release from irradiated cells that acted on bystander cells.

Journal: Oncotarget

Article Title: Dual effects of radiation bystander signaling in urothelial cancer: purinergic-activation of apoptosis attenuates survival of urothelial cancer and normal urothelial cells

doi: 10.18632/oncotarget.21995

Figure Lengend Snippet: Summary schematic diagram of the radiation-bystander effect that is common to urothelial cancer (T24) cells and normal urothelium (SV-HUC). Directly irradiated cells signal to bystander cells activating pro-apoptotic signaling mechanisms and causing DNA double stand break damage resulting in decreased cell survival. The bystander effect studied here was mediated by ATP release from irradiated cells that acted on bystander cells.

Article Snippet: Urothelial carcinoma cells T24 (McCoy’s 5A) [ ], HT1376 MEM) [ ], immortalised normal urothelial cells SV-HUC (F12K) [ ], were purchased from ATCC; primary normal HUC (Urothelial Cell Medium) were obtained from ScienCell Research Laboratories.

Techniques: Irradiation